慢性前脑缺血大鼠学习、记忆功能的研究

Study on learning and memory ability in cbronic forebrain ischemia rats

摘 要：目的 研究慢性持续性脑血流量下降对大鼠学习、记忆功能的影响。方法 采用双侧颈总动脉永久结扎方法制备慢性前脑缺血动物模型；利用激光多普勒血流仪检测各组大鼠术后不同时间点（术后24h、7d、15d、30d、60d、90d、120d）额叶皮质、海马区局部脑血流量（rCBF）；采用被动回避性条件反射──跳台试验检验各组大鼠（时间点同前）学习能力；利用水迷宫方法检验各组大鼠记忆功能。结果 大鼠术后额叶皮质、海马区的rCBF明显下降，以术后24h最明显，至术后120d时仍明显低于正常，呈慢性持续性下降的趋势。同时各实验组大鼠学习、记忆能力也明显下降，且有随时间推移而逐渐加重的倾向。结论 慢性持续性脑血流量下降可导致实验大鼠出现进行性认知功能障碍。

Abstract: Objective To study the learning and memory ability after chronic cerebral hypoperfusion. Methods We adopted the permanent occlusion of bilateral common carotid arteries in Wistar rats to produce the chronic forebrain ischemic; The laser Doppler was used to assess the cerebral blood flow,We estimate the performance of the rats in the Morris water maze and other tests. Results After the operation,the rCBF of the frontal lobe and hippocampal were decreased sharply,ard the reduction is progressively; The rats showed progressive cognitive im-pairmert after the operation. Conclusion The rats showed progressive cognitive inpairment following chronic cerebral hypoperfusion.