宫颈癌患者HPV16感染及其血清抗体检测分析

The significance of serum HPV16 antibody in the cervical cancer cases of infected by HPV16

目的 :探讨宫颈癌与人乳头瘤病毒 16 (HPV16 )感染及其血清抗体的关系 ,为HPV感染及宫颈癌的临床诊断提供理论依据。方法 :选择 2 8例宫颈癌患者 ,用PCR方法检测宫颈癌组织的HPV型别 ;并采用重组杆状病毒 昆虫细胞系统制备HPV16病毒样颗粒 (VLPs) ,用ELISA方法检测其血清HPV16VLPs抗体 ,同时以 36份尖锐湿疣患者血清及 72份健康体检者血清作为对照。结果 :宫颈癌HPV通用型DNA阳性率为 5 0 %(14/ 2 8) ,HPV16DNA阳性率为 42 .9% (12 / 2 8) ,HPV18DNA阳性率为 3.5 % (1/ 2 8)。宫颈癌组血清HPV16VLPs抗体阳性率为 42 .9% (12 / 2 8)、抗体滴度中位数为 0 .0 380 (0 .0 45 2 ) ,较健康对照组阳性率为 1.4% (1/72 )、中位数 - 0 .0 2 2 0 (- 0 .0 2 6 5 )和尖锐湿疣组阳性率为 8.3% (3/ 36 )、中位数为 0 .0 16 5 (0 .0 415 )差异具显著性意义 (P <0 .0 1)。HPV16DNA检测法与HPV16VLPsELISA血清抗体检测法两者间呈中度相关 (K =0 .471,P<0 .0 5 )。结论 :本组宫颈癌以HPV16感染为多见 ,HPV16VLPs血清ELISA抗体检测可用作HPV16感染的血清学诊断和宫颈癌的免疫学研究。

Objective: To observe the relationship of cervical cancer and human papilloma virus 16 infection and the titer of serum HPV16 antibody in southern of zhejiang province P.R.China,so as to assist the diagnosis and further research of cervical cancer.Methods: 28 cases of cervical cancer tissues typed the HPV16 by PCR. The control groups were the serum of 36 cases of genitalia warts and 72 health women. To test the serum concentration of HPV16 antibody by ELISA(the antigen use the pure HPV16 Virus-like-particles).Results: The general tissue type of HPV DNA positive rate is 50%(14/28),HPV16 DNA positive is 42.9%(12/28) and HPV18 DNA positive is 3.5%(1/28) in the group of cervical cancer. The titer of the serum HPV16 antibody is MD=0.0380(0.0452) in the group of cervical cancer,and is higher than the group of genitalia warts MD=0.0165(0.0415) (n=36) and the group of health women MD=-0.0220(-0.0265) (n=72) (P <0.01). There is moderate correlation between the methods of tissues HPVDNAPCR and serum HPV16 antibody(K=0.471,P <0.05).Conclusion: Most common HPV infection with cervical cancer is type 16;the serum titer of anti-HPV16 may become a diagnostic laboratory index for HPV16 infection and cervical cancer.