LAK细胞对人肝癌细胞的杀伤及电镜观察

MORPHOLOGICAL OBSERVATION OF HUMAN LAK CELL KILLING ACTIVITY ON H_(7402) CELLS

本文观察了淋巴因子激活的杀伤细胞(LAK)在体外对人肝癌细胞株(H_(7402))的杀伤活性及透射电镜下LAK细胞对H_(7402)的杀伤作用.实验证实:LAK细胞对人肝癌细胞H_(7402)有较强的杀伤活性;而对正常人外周血淋巴细胞无杀伤作用;透射电镜下:LAK细胞与H_(7402)细胞共同孵育30分钟,可见二种细胞各自伸出突起,互相接触.共同培养4小时,可见,H_(7402)细胞呈“凋零”坏死.

We studied morphological observation and cytotoxicity of Lymphokine-Activated Killer (LAK) cell killing H7402 cells. A 4 hours 51Cr release assay was used to measure LAK cell killing of tar get cell. The results confirm that LAK cell can kill H7402 in vitro. Under transmission electronmi-croscope,30 minutes after co-culture,close binding with many interdigitations and small extracellular spaces between LAK cells and H7402 cells bound with LAK cells developed apoptotic cell death.