肝再生刺激因子、胰岛素和胰高血糖素对体外肝细胞增殖的作用

EFFECT OF HSS, INSULIN AND GLUCAGON ON HEPATOCYTE REGENERATION IN VITRO

本研究采用体外细胞培养分组对比法检测肝再生刺激因子(HSS)、胰岛素或/和胰高血糖素对大鼠原代培养肝细胞和人肝肿瘤细胞株HepG_2 DNA合成(肝细胞再生)的作用。结果发现,HSS无论对大鼠肝细胞或人肝肿瘤细胞均具有较强的刺激DNA合成的作用(与对照组相比分别增加1.14和1.05倍,P<0.01)。而对人纤维母细胞则无促进DNA合成的作用。低浓度胰岛素或/和胰高血糖素对肝细胞DNA合成无作用。高浓度胰岛素(10^(-5)M)具有中等度促进肝再生的作用(与对照组相比增加40％,P<0.05)。两者联合应用(G+I)似无协同作用。据此,我们认为,HSS是一种潜在的促进肝再生的有效因素,可能具有临床实用价值。体外应用优于现在临床广泛应用的胰岛素加胰高血糖素(G+I)疗法。

This experiment was designed to confirm that Hepatic Stimulator Substance (HSS).Insulin or/and Glucagon addition stimulated DNA synthesis(Hepatocyte Regeneration)in primary hepa-tocyte cell cultures prepared from adult rat liver and in human hepatoma cell line, Hep G2, in vitro. The results showed that HSS could strongly stimulate DNA synthesis in both of rat hepatocyte and hepatoma cells (Hep G2) (Compared with control, DNA synthesis increased 1. 14 and 1. 05 times, respectively. P<0. 01). But no effect on fibroblast. However,Insulin or/and Glucagon at low concentration could not stimulate DNA synthesis in hepatocyte or Hep G2. High concentration of Insulin(10-5M) can moderately increase DNA synthesis (Compared with control 40% increase, P<0. 05). Therefore,we concluded that HSS is a potential factor which can stimulate liver regeneration. Perhaps,it can be used in clinic. It may be better than the therapy of Insulin plus Glucagon which is widely used in clinic recently.