大肠癌组织中表皮生长因子及受体的检测

DETECTION OF EPIDERMAL GROWTH FACTOR AND EPIDERMAL GROWTH FACTOR RECEPTOR IN COLORECTAL CANCERS

本文应用免疫组化ABC法检测52例大肠癌和18例正常结肠组织中表皮生长因子(EGF)和表皮生长因子受体(EGF-R)的表达状况。正常组织中EGF阳性率22.2％,EGF-R阳性率16.7％,大肠癌EGF阳性率67.3％,EGF-R阳性率61.5％,二者均明显高于正常对照组织,(P<0.05)。EGF和EGF-R阳性率与患者年龄、性别及肿瘤部位无明显相关,但随着肿瘤浸润度的加深,EGF与EGF-R的阳性率逐渐增高,有淋巴结转移者二者阳性率高于淋转阴性者,特别是EGF与EGF-R双阳者中有82.6％为进展期大肠癌,另发现低分化大肠癌中EGF和EGF-R阳性率明显低于中高分化癌。本文结果提示:部分大肠癌存在EGF或EGF-R的过度表达;EGF与EGF-R的过度表达与肿瘤浸润度及淋巴转移有关,其检测可作为诊断肿瘤恶性程度的一项辅助指标;部分正常大肠粘膜组织中也有少量EGF或EGF-R表达。

We immunohistochemically examined expression of EGF and EGF-R in 52 colorectal cancer tissues and 18 normal colon tissues. EGF and EGF-R positive rate were 22. 2% and 16. 7% in normal colon tissues,67. 3% and 61. 5% in colorectal cancers, The positive rate of EGF and EGF-R in colorcetal cancer significantly higher than that in normal tissues (P<0. 05). the positive rate of EGF and EGF-R in advance cancers were higher than in early stage cancer. Expression of EGF or EGF-R was no relation to age,sex or location of cancer, Our results suggests that part colorectal cancer have overexpression of EGF and EGF-R,EGF and EGF-R autocrine may contribute to the growth of colorectal cancer.