Ag－NOR计数在判断大肠癌预后中的价值

Prognostic Value of Nucleolar Organizer Regions in Colorectal Carcinoma

应用胶质银染技术对５６例根治性手术切除的大肠癌标本进行了定量观察。结果发现，Ａｇ－ＮＯＲ计数与患者的存活时间呈负相关（ｒ＝－０．７７Ｐ＜０．００１）。Ａｇ－ＮＯＲ＜４个／核，５年累积生存率是８０％，Ａｇ－ＮＯＲ４～６个／核为１５％，＞６／核为零。对数秩时序检验Ｐ＜０．００１。恶性程度低的肿瘤其颗粒分布以中央型为主，似恶性程度越高颗粒分布越弥散。大肠癌细胞核Ａｇ－ＮＯＲ计数与ＤＮＡ含量有一定相关性。多因素生存分析显示Ａｇ－ＮＯＲ计数是大肠癌一个重要预后指标。

NORs were quantified in a series of 56 colorectal carcinoma of radical resection using the silver-colloid method. The results showed that there was a negative correlation between mean counts of Ag-NOR and patients survival time (r=-0.77,P<0.001). From the Kaplan-Meier survival curves,we could know that the 5-year survival rates of Ag-NOR (<4.4～6,>6) were 80%,15%, and 0,respectively (P<0.001).Ag-NOR was mainly located in the center of nucleus in low malignant tumor and scattered in the nucleus in high malignant tumor. There was a correlation between Ag-NOR counts and DNA content.The result of a multivariate regression analysis documented that Ag-NOR counts was an important prognostic parament.