双丁酰环腺苷酸对体外HT29细胞增殖的调节

Regulation of Proliferation of Human Colon Adenocarcinoma Cell Line (HT 29) by db-cAMP

采用酸性磷酸酶细胞原位计数及流式细胞仪（ＦＣＭ）技术，观察了双丁酰环腺苷酸（ｄｂ－ｃＡＭＰ）对体外人结肠腺癌细胞系（ＨＴ２９）增殖的影响。结果：ｄｂ－ｃＡＭＰ在１０－４～１０－９Ｍ范围内可促进细胞增殖，尤其在１０－６～１０－８Ｍ时，ｄｂ－ｃＡＭＰ组ＯＤ值分别为１．５０±０．１１，１．５８±０．０７，１．５７±０．１２，分别为对照组的１０．３０％，１６．１８％和１１．７６％，差异显著（Ｐ＜０．０５或０．０１）。ｄｂ－ｃＡＭＰ浓度为１０－３Ｍ时，则抑制细胞增殖，至培养第５天，ｄｂ－ｃＡＭＰ组ＯＤ为１．０５±０．１３，对照组为１．２３±０．１７，有显著差异（Ｐ＜０．０５）。ＦＣＭ结果提示，ｄｂ－ｃＡＭＰ对细胞的抑制作用，与延长细胞Ｇ１／Ｓ期有关。结果表明，ｄｂ－ｃＡＭＰ对ＨＴ２９细胞增殖有双向调节作用。作者还对其作用机制进行了探讨。

The purpose of this investigation was designed to elucidate the role of dibutryryl cAMP (db-cAMP) on the control of HT 29 cells proliferation with the methods of cell-counting in situ by acid phosphatase assay and cell cycle analysed by flow cytometry (FCM).The results showed that low concentration of db-cAMP (in the range of 10-4 M to 10-9M) promoted cell proliferation. While the concentration of db-cAMP was 10-6 M,10-7 M,10-8M,the OD values were 1.50±0.11,1.58±0.07,1. 52±0.12,which were 10.13%,16.18%,11. 26% as high as that in control group,respectively (P<0. 05 or 0.01).However,cell proliferation significantly reduced by 10-3 M db-cAMP in the 5th culture day,and the OD value in db-cAMP group (1. 05±0. 13) was much less than that in control group (1.23±0.17) ,P<0. 05). Cell cycle analysis indicated that cell cycle of HT 29 cells was delayed in G1/S phase by 10-3M db-cAMP. Our findings suggest that db-cAMP had bidirectional control effects on the proliferation of HT 29 cells,and the mechanisms has been discussed.