树鼩对人乙型肝炎病毒实验感染的量反应

Doses-response of Human Hepatitis B Virus Infection in Tree Shrews

５０只树分成５组。将ＨＢＶ含量为１０~８ＣＩＤ／ｍｌ的美国ＮＩＨ提供的标准血清稀释成１０^（－６）～１０^（－１０）等５个浓度，先经股静脉分别给各组每只动物接种０．５ｍｌ。３天后，经腹腔再注射等量同样稀释血清一次。实验观察１６周。经血清学及免疫组织化学检测，５个稀释度的血清引起树实验感染率依次为８０．１％、８８．８％、６６．６７％、５５．５６％及４２．８％。表明：树对人ＨＢＶ的易感性与黑猩猩近似。由于其体形小，易于饲养管理，是研究与ＨＢＶ感染有关的疾病、治疗药物及疫苗质量检测的较好的实验动物。

The standard serum containing HBV concentration with 10￣8 chimpanzee infectious doses per milliliter (CID) were provided by National Institute Health of United States. This serum was gradually diluted from 10￣1 to 10￣10 dilutions,the 10-8 dilution equal one CID HBV.50 Tree Shrews were divided into five groups, and each group was 10 animals. These groups were inoculated with 10-6, 10-7, 10-8, 10-9, 10-10 dilutions serum respectivily. Each animal was injected with diluted serum 0. 5 ml by i. v. and 0. 5 ml by i. p. Then,the animals blood were drawn from femoral vein weekly for detecting the HBV infectious markers with ELISA,and the HBsAg in liver cells of experimental Tree Shrews were detected by immune histochemic PAP method. The results showed that the infectious rates of HBV in five groups were 80. 0%, 88. 9%, 66. 7%, 55. 6% and 42. 9% respectivily,showing a dose-response relationship. These results suggest that tree shrews are sensitive to HBV infection and their susceptibility is similar to that of chimpanzee.