免疫荧光法检测流行性出血热IgG的应用

Clinical Application of Detecting Anti-epidemic Hemorrhagic Fever Virus IgG by Immunofluoresence Technic

报道免疫荧光法检测流行性出血热IgG在临床的应用。结果:临床诊断为出血热621例、可疑出血热118例、发热待查25例、肾脏疾患7例,其IgG阳性数分别是511例(阳性率82.28％)、59例(50.00％)、9例(36.00％)及5例(71.42％);患者男多于女,好发于21～40岁,幼儿亦有感染;发病5天内即可检出抗体,且随病日延长抗体滴度相应升高,至发病第11～15天抗体滴度≥1/640者占44.61％,第16～20天增到60.00％,本方法简单易行,且符合率较高,可作为早期诊断的依据之一。

The clinical application of immunofluoresence determination of anti-epidemic hemorrhagic fever viruses was investigated, of the seven hundred and seventy one specimens in total, 621 were clinicallydiagnosed as epidemic hemorrhagic fever (EHF). 118 as EHF dubious ,25 as fever of undetermined origin (FUO) and 7 as renal desease. The Positive rates of anti-EHFV IgG in them were 511 (positive rate : 82.28% ) ,59(50%),9(36%) and 5(71.42%)respectively. Malcs were more easily infected than females, and higher incidence was found in the age groap from 21-40 years .though infants might be infected too. The corresponding antibody could be detected in 5 days after the onset and the Ab titer increased with the course of the disease. The Ab titer ≥ 1/640 was shown in 44.61% patients on the 11-15th day and in 60% on the 16-20th day. This method can be regarded as one of the bases of early diagnosis of EHF because of its simplicity.convenience and high coincidence rate.