肺癌患者血清可溶性白细胞介素-6受体和一氧化氮检测的临床意义

Clinical Value of Determination of Serum Soluble lnterleukin-6 Receptor and Nitric Oxide in Patients with Lung Cancer

目的:评价可溶性白细胞介素-6受体(sIL-6R)与一氧化氮(NO)水平对肺癌诊断和病情评估的临床价值。方法:用双抗体夹心酶联免疫吸附试验(ELISA)检测了20例正常对照和60例肺癌患者发病不同阶段,及化疗前、后血清中sIL-6R水平,及光度法检测NO水平。结果:肺癌患者血清中sIL-6R水平高于正常对照组,IV期肺癌组血清中sIL-6R水平又高于Ⅰ期肺癌组。化疗后,肺癌患者血清中的sIL-6R水平明显下降,不但低于化疗前水平,更低于对照组水平。肺癌患者血清中NO水平也低于正常对照组。结论:肺癌患者NO水平下降,提示肺癌局部抗肿瘤功能可能存在缺陷。肺癌患者体内存在sIL-6R的高表达,随着肺癌病情进展sIL-6R表达增多,sIL-6R的测定有助于临床肺癌发病的辅助诊断,并且对肺癌分期和预后判断有一定的临床意义。经直线相关性分析未发现血清sIL-6R水平与NO间有明显相关性。

Objective: To evaluate the value of the levels of soluble interleukin-6 receptor (sIL-6R) and nitric oxide (NO) in the diagnosis and state of illness in patients with lung cancer. Methods: The levels of serum sIL-6K were determined by enzyme linked immunosorbent assay (ELISA) in 20 cases with normal control, and 60 cases with lung cancer in different phases and chemotherapy. The levels of NO were detected by spectrophotometry. Results: The levels of sIL-6R in patients with lung cancer were higher than those of slL-6R in stage I. After chemotherapy the levels of sIL-6R were lower than that before the treatment, and even lower than those in normal control. Conclusion: The levels of NO decreased in patients with lung cancer, and it indicated that there were some defections in local anti-tumor functions of lung cancer. The high expression rates were found in patients with lung cancer, and the levels of sIL-6R went up with the development of illness. sIL-6R was useful (or clinical stage and prognosis. There was no significant correlation between NO and sIL-6R by linear correlation analysis.