老年高血压病合并冠心病、脑梗塞患者AT1R基因多态性的研究

Influence of angiotensin II type 1 receptor gene polymorphism on the elderly hypertensive patients

目的 探讨血管紧张素 II- I型受体 (AT1 R)基因 A1 1 66/ C多态性与老年原发性高血压病的关系 ,并探讨原发性高血压病的发病机制。方法 应用聚合酶链式反应、限制性内切酶酶解 (PCR- RFLP)的方法检测 40例健康人和 92例原发性高血压病患者 (其中 31例合并冠心病 ,37例合并脑梗塞患者 )的 AT1 R基因型 ;生化技术测定血脂水平。结果 原发性高血压病组、合并冠心病组及合并脑梗塞组的 C等位基因频率 1 4 .6%、1 4 .5%和 1 0 .8% ,分别显著高于正常对照组的 3.7% (P<0 .0 5) ;带有 C等位基因的原发性高血压病患者无论是否合并冠心病、脑梗塞 ,其血浆 LP(a)水平均增高。结论 提示 AT1 R基因可能是老年原发性高血压病的重要遗传因素 ,AT1

Objective To identify the A1166/C polymorphism of angiotensin II type 1 receptor(AT1R)Gene in the elderly hypertension complicated with coronary atherosclerotic heart disease(CHD) and with brain infarction(BI). Methods AT1R genotyping with polymerase chain reaction and digestion of restriction enzyme was performed in 40 normotensive subjects, 24 hypertensive patients without cardio-cerebrovascular diseases, 31 hypertensive patients with CHD and 37 hypertensive patients with BI .The relationship between the polymorphism of AT1R gene and plasma lipid levels was also studied.Results The results showed the frequencies of C allele among the three groups of hypertension were higher than those among the health controls, respectively(P<0.05). Among the three groups, the plasma LP(a) levels in AC patients were significantly higher than those in AA patients (P<0.05), but not in controls.Conclusions AT1R gene contributes to the development of the elderly hypertension and could modulate the effects of lipids on large arteries.