地龙药材两种采收加工方法的比较

Comparison on the in vivo activity of earthworm colleceted and processed by two methods

目的 对地龙药材的采收加工方法进行改进 ,并和药典方法进行比较。方法 用纤维蛋白平板法和TAME法测定两种采收加工方法所得地龙的纤溶活性及单位效价 ,采用不同干燥条件对地龙进行干燥 ,并给小鼠灌胃两种地龙提取液 ,测出最大耐受量。结果 改进新法采收加工地龙药材活性较大 ,两种采收加工方法的毒性无显著性差异 ;在日光下暴干省时、质量好、收率高。结论 改进新法采收加工地龙药材与药典方法相比其活性较高 ,并不增加药材本身的毒性 。

OBJECTIVE: To compare the rationality between the new method and the pharmacopoeia method of earthworm collected and processed. METHODS: The fibrin dissolution activation and unit titre were measured by fibrin plate and TAME methods. The maximum toleration was assessed after oral administration to mice of the earthworm extracts by the two methods. RESULTS: The new experimental method showed greater activation and the both methods showed no obvious difference of their toxicity. The exposure to sunlight saved time and retained quality. CONCLUSION: The new experimental method retained higher activation than pharmacopoeia method without increase of its toxicity. Exposure to sunlight is recommendable.