超声波对小白鼠生精上皮的影响

EFFECTS OF ULTRASOUND ON SPERMATOGENIC EPITHELIA IN MICE

用功率为５Ｗ／ｃｍ２、频率为１．１０ＭＨｚ的超声波照射小白鼠睾丸５分钟（实验组１）、１０分钟（实验组Ⅱ），分别于处理后２４小时，４８小时及７天时间切取睾丸组织，制作石蜡切片，在光镜下观察生精上皮的组织学变化并与对照组进行比较。结果显示：（１）小白鼠睾丸经超声波照射后，曲细精管萎缩，管径变小；生精上皮变薄，精子发生时相消失；生精细胞减少，没有精子形成。（２）超声波照射１０分钟对生精上皮的损伤比照射５分钟更为严重。（３）超声波照射后２４小时，生精上皮即受到破坏，精子细胞减少；照射后４８小时，上皮受损伤的程度增大；照射后７天时间，曲细精管的组织学结构开始恢复，但仍无精子形成。（４）超声波对生精上皮的影响主要限于精母细胞、精子细胞和精子，而精原细胞与支持细胞没有明显变化。上述结果表明，超声波能够抑制小白鼠的精子发生，该抑制作用可能可逆。

The testes in scrota of mice were irradiated by ultrasound with a power of 5 W/cm2 and a frequency of 1. 10 MHz for 5 minutes(experiment gorupⅠ) and 10 minutes (experiment group Ⅱ) separately. Histological sections of the animal testes of the experimental groups were prepared after 24 hrs, 48 hrs, and 7 days of the ultrasound treatment respectively.Light microscopic structures were observed and compared between the control and the experimental groups. Results displayed that : (1) Seminiferous tubules from the ultrasound treated testes atrophied with the tubule diameter shrunk; Spermatogenic epithelia of the experimental animals got thinner with the spermatogenic stages absent; Spermatogenic cell numbers reduced with no spermatozoon developed. (2) Ultrasound in the present dose did more serious damages to the spermatogenic epithelia when applied for 10 minutes than that for 5minutes. (3) From 24 hrs to 48 hrs after the ultrasound treatment, damages to the spermatogenic epithelia became more significant. After 7 days of the irradiation, the histological structures of the spermatogenic epithelia showed some recoveries. No sperm developed. (4)Spermatogenic cells affected by the ultrasound contained mainly spermatocytes , spermatids,and spermatozoa. However, spermatogonia and the Sertoli cells manifested no obviously microscopic structure changes. All the above- mentioned results indicate that ultrasound could suppress the spermatogenesis in mice, and that the effects may be reversible.