马桑内酯致癫痫大鼠黑质网状部的形态计量研究

A Morphometric Study on Subtantia Nigra Pars Reticulata of Epileptic Rats Induced by Coriaria Lactone

用光镜和电镜定量研究了马桑内酯致ＳＤ大鼠急性局灶型癫痫发作１小时后黑质网状部的结构变化．与对照组（５只）比较，在实验组（５只）脑的重量、体积及黑质横切面积均无明显改变的前提下，实验组黑质网状部单位面积内神经元数量，神经元胞体、胞核的直径以及神经元胞体、胶质胞体、血管和神经毡的面积分数均无显著性变化，表明实验组黑质网状部神经元的数目及大小没有显著性变化，实验组神经元胞体内粗面内质网、游离核糖体的面积分数以及游离核糖体的数密度显著减少，神经元胞质基质的面积分数则显著增加，线粒体、高尔基复合体和溶酶体的面积分数无显著性改变。马桑内酯所致急性局灶型癫痫发作１小时．黑质网状部神经元的粗面内质网和游离核糖体的显著减少，表明该神经元合成蛋白质的活性有所减弱。本文对该结果的意义进行了讨论。

This experiment was carried out with ten adult male SD rats, weighing 200～250g,5 for experimental group,other 5 as control.The epileptic activity was produced by Coriaria Lactone in experimental group and the control animal replaced by saline solution.The substantia nigra pars reticulata(SNR)was studied quantitatively after seizures for lh.The area of substantia nigra,numerical density of SNR neurons,and the mean diameter of neuronal soma and it's nucleus were measured directly under light microscope.There was no obvious change in experimental group as compared with control.The areal fraction of SNR neuronal soma,neuroglial soma, blood vessels and neuropil were measured stereologically from sections.There was no difference between two groups.The areal fraction of rough endoplasmic reticulum,mitochondria,Golgi complex,lysosome,free ribosome and cytoplasmic matrix were measured stereologically from electron microscopic films and the numerical density of free ribosome was counted also;The positive changes were the decrease significantly in the areal fraction of rough endoplasmic reticulum and free ribosomes and the numerical density of free ribosome.For the volume of substantia nigra,the size and number of SNR neuron were no obvious changes in experimental rat,these changes of experimental SNR neurons suggest that the protein synthesis activity in SNR neurons decreased.