糖尿病妊娠胎鼠胰岛β细胞的形态学和体视学研究

Morphological and stereological studies of the islet β cell in Fetuses of Diabetic Mother Rats

本实验选用雌性Wistar大鼠，妊娠第一天腹腔注射四氧嘧啶（15mg／100g体重）。分别于妊娠第17、18、19、20天剖腹取胎胰，一部分用改良Bouin液固定，石腊包埋切片，HE染色及免疫组织化学PAP法显示β细胞，并测定β细胞的数面密度和体密度；另一部分常规电镜制片及观察。结果显示，实验组β细胞的数面密度均显著高于对照组，第17、18天实验组胎鼠β细胞分泌尿粒校对照组的增大，数量增多。而17天胎鼠胰岛为非常不规则的内分泌细胞团，对胰岛素抗血清呈阳性反应的细胞少，阳性反应弱。随着胎鼠的生长发育胰岛细胞逐渐聚集成条索状或团块状，阳性细胞增多，阳性反应渐增强。上述结果提示：妊娠高血糖可以促进胰岛β细胞数量增多，分泌颗粒成熟。

Female Wistar rats received intraperitoneal lnjection of a1loxan(15mg/100g weight)after pregnancy. The animals were killed on the 17, 18, 19, 20 day of pregnancy and the pancreas of fetuses were observed by light and electronic microscope. The islet 5 cells were showed by the immunohistochemistry (PAP method) and NA and Vv of 9 cell were measured. There were a definite rise for NA of 5 cells in fetuses of diabetic mother rats and their secretory granules were more and bigger than control group. In the meantime,the endocrine cells of pancreas gathered to form glomerulosa-like or fasciculata-like structure from 17 to 20 day of pregnan-cy. It is concluded that the maternal diabetes could accelerate the incfease of β cell number and the maturation of it's secretory granules.