摘要
本文用白细胞共同抗原（CD45）和巨噬细胞特异性抗原（CD68）的单克隆抗体，对24、28、30、33、36周胎龄及正常成人的视网膜进行标记。结果表明幼稚的阿米巴型、成熟的分支型和介于两者之间的中间型（依突起数可分为单极、双极、多极形的小胶质细胞）小胶质细胞均表达CD45；除分支型外，其余的小胶质细胞亦同时表达CD68。小胶质细胞分布于视网膜的内核层、内网层、节细胞层、在其分布的层内，细胞分布均匀．相邻的细胞之间无接触。节细胞层的细胞数量比内核层、内网层多．但内核层、内网层的细胞比节细胞层的细胞幼稚，形态上表现为胞体较大，细胞无分支或分支较少．无次级突起.同时发现随胎龄的增大．细胞数量增多，突起增多并变长变细，36周胎时已分化为分支型小胶质细胞。亦观察到血管周小胶质细胞和血管旁小胶质细胞。在成体视网膜小胶质细胞以分支型为主，同时也存在一系列形态较幼稚的CD68阳性细胞。
The retinas of 24，28，30，33，36 fetal week and normal adult human were labeled with monoclonal antibody against human leukocyte common antigen CD45 and macrophage antigen CD68． The results showed that the antibody of CD45 could label primitive amoeboid，mature ramified and intermediate differential microglial cells（monopolar， bipolar， multipolar type），these glias also could be stained by the antibldy of CD68， except ramified microglia．Microglial cells distributed in the ganglion cell layer（GCL）， the inner plexiform layer （IPL） and the inner nuclear laver（INL）．A regular mosaic-like arrangement of microglial cells in their distributive lamellae was evident． with no overlapping between adjacent cell terriotories． The population of microglias in the GCL was more than that in the INL and IPL，and the processes of cells of the former were more than those of the latter． With the development of the fetal age．the amount and bronches of microglias increased．At 36 fetal week，ramifed microglias aoperaed． The perivascular and juxtavascular microglia were also observed． In the adult retina， the ramified microglial cells predominated than the juvenile CD68－positive cells．
出处
《四川解剖学杂志》
1998年第2期99-103,共5页
Sichuan Journal of Anatomy
