葡萄糖、精氨酸对新生大鼠胰岛β细胞群的影响

The Effect Of Glucose And Arginine On Pancreatic β Cell Mass in Neonatal Rat

为研究葡萄糖、精氨酸对新生大鼠胰岛β细胞群的影响，选用Ｗｉｓｔａｒ新生大鼠，于出生后第２日起腹腔注射葡萄糖加精氨酸，共６ 天。分别于出生后第１０、２０ 天剖腹取胰腺，进行ＨＥ及胰岛素免疫组织化学（ＰＡＰ）染色，并应用图像分析系统测量胰岛及β细胞群的直径。结果：实验组和对照组大鼠生后２０ 天胰岛直径较其第１０ 天的显著缩小，对照组β细胞群的直径从第１０ 天到２０ 天，也表现为明显缩小；然而，实验组β细胞群直径却表现为有意义的增加。

For investigation the effect of glucose and arginine on pancreatic β cell mass, newborn rats were treated with glucose and arginine(G+A) for 6 days and were killed on the tenth and twentieth day after birth. The β cell were showed by immunohistochemistry(PAP) method and the Medical Image Processing System was used to measure the diameter of Langerhans islands and the β cell mass. Results: The diameter of islets in G+A and control group on the twentieth day were definite decrease,and a similar change occurred at the diameter of the β cell mass in control group. But there was a definite increase in diameter of β cell mass of G+A group on the twentieth day. The results indicate that glucose and arginine may promote the growth of β cell mass.