体外人脐静脉内皮细胞分泌IL—6的研究

Study on IL-6 Secreted by Endothelial Cells in Vitro

内皮细胞是一个非造血细胞 ,却表达和 /或产生大多数已知的造血受体和细胞因子。这些因子和受体的生物学作用仍不十分清楚。目的 :本研究旨在探求内皮细胞自然产生 IL- 6及其生物学功能。试图阐明 IL- 6在造血调控中的作用。在建立人脐静脉内皮细胞体外培养模型的基础上 ,收集原代培养内皮细胞 1 - 9天培养上清液 ,离心 ,- 2 0℃保存备用。采用双抗夹心 ELISA法对上清液中 IL- 6进行定性和定量测定。结果 :在未加任何刺激因素的条件下 ,从培养的人脐静脉内皮细胞培养上清液中可测及 IL- 6(735.380 1± 883.53pg/ ml)。随着内皮细胞培养天数的增加 ,IL- 6的释放量也随之增加。在第 6天时接近释放峰值 IL- 6(2 80 8.61 pg/ ml)。结论 :体外培养的人脐静脉内皮细胞具有自然分泌 IL- 6的功能。 IL- 6对各系造血祖细胞的增殖均起促进作用。本研究为内皮细胞支持各系造血干 /祖细胞的增殖和分化提供了理论依据。

WT5BZ]Endothelial cells which are not nonhemopoietic cells express and/or secrete most of the hematopoietic receptors and cytokines whose biological role is still poorly understood. To discuss the function of IL 6 which was produced by endothelial cells on regulation of hematopoiesis. Human umbilical vein endothelial cells culture model was founded.The human endothelial culture supernatant(HECS)which was harvested from primary culture medium for 1～9 days and was centrifuged and stored at 20℃. IL 6 in HECS was measured using sandwich enzyme linked immunosorbent assay(ELISA) method.Results:IL 6 was tested(IL 6:735.3801±883.53pg/ml) in HECS with no any stimulated factors on endothelial cells culture. The amount of IL 6 increases along with the cell culture time. At 6th day the amount reaches its peak (2808.61pg/ml). Conclusion:Human umbilical vein endothelial cells cultured in vitro can secrete IL 6 spontaneously. IL 6 can promote the colony formation of granulocytic,erythrioid,meakarycytic and multipotential stem cells and progenitor cells.