摘要
从人基因组 DNA获取神经营养素 - 4(neurotrophin- 4 ,NT- 4 )基因 ,并对该目的基因进行序列测定 ,本实验直接从人脑组织中提取基因组DNA,根据人神经营养素 - 4的 c DNA序列 ,设计一对寡核苷酸引物 ,采用 PCR,获得人 NT- 4基因 ,用DNA序列分析 NT- 4基因。结果 ,我们获取的人NT- 4基因与文献报道有五个碱基不同。我们认为 :直接采用 PCR来获取目的基因 ,出现碱基错配的可能性较大 ;测序仪器和实验条件对结果有一定影响关系。
According to the cDNA sequence of human neurotrophin 4(NT 4),a pair of Oligonucleotide primer were synthesized for to amplify the NT 4 gene directly from human genomic DNA and to analyze it′s sequence. Amplification was performed by PCR directly from human genomic DNA. The product of amplification were sequenced by automatic sequencer. We fonnd that the DNA sequence of this human NT 4 genes was some what different in five base pairs from the published data. We suggest that the difference may be caused by the process of PCR
出处
《四川解剖学杂志》
2000年第3期133-135,共3页
Sichuan Journal of Anatomy
