2,4-二硝基酚所致小鼠发热模型的建立及阳性解热药物的选择

Establishment of fever model in mice and choice of positive control antipyretics

目的 :建立一种简便的小鼠发热模型 ,使之应用于解热药物药效初筛。方法 :12 ,4-二硝基酚 14mg/ kg、2 8mg/ kg和 35 mg/ kg皮下给药后 ,分别于 0 .5、1.0和 1.5小时三个时间点测定小鼠体温。 2在造模前 0 .5小时 ,口服乙酰水杨酸 0 .8g/ kg和腹腔给予安痛定 (按所含氨基比林 2 0 mg/ kg给药 ) ,并于造模后 0 .5、1.0和 1.5小时测定小鼠体温。结果 :2 ,4-二硝基酚可引起小鼠发热 ,升温 1℃左右。乙酰水杨酸作为阳性对照药应用于此模型引起大量动物死亡。安痛定的解热作用肯定。结论 :小鼠应用 2 .4-二硝基酚可建立化学性发热模型 ,但升温持续时间短暂 ,对作用快速且强大的解热药物药效初筛具有应用价值 ,乙酰水杨酸不能作为阳性对照药应用于此模型。

Objective: To establish in mice a simple fever model used in screening antipyretics. Methods: (1) 0.5h, 1.0h and 1.5h after administration of 2,4- Dinitrophenol, the body temperature was measured. (2)Acetylsalicylic acid (0.8g/kg, po.) and Antondini( equivalent of 20mg/kg Aminopyrine contained, ip.) were given 0.5h before injection with 2,4-Dinitrophenol and the body temperature was measured 0.5h, 1.0h and 1.5h after the injection. Results: 2,4- Dinitrophenol was able to induce fever, raising 1℃ or so. Acetylsalicylic acid was able to induce death of large quantities of model mice. Antondini had a definite antipyretic action in such a model. Conclusion: 2,4- Dinitrophenol can be used to establish a chemical fever model in mice, but it is only available for screening the potent antipyretics because of the short duration of the raised temperature. Acetylsalicylic acid cannot be used as positive control drug in this fever model.