花生编码磷脂酶D的类PLD基因的鉴定和部分测序(英文)

Identification and Partial Sequence of a PLD-like Gene Encoding for Phospholipase D in Peanut

花生工业界认为收获前花生黄曲霉毒素的污染是全世界花生工业界面临的最严峻的挑战。干旱胁迫是加重花生黄曲霉真菌侵染和毒素污染最重要的环境因素。选育花生抗性品种将使美国花生工业处于优势地位。在这一研究报告中 ,我们鉴定出了一个新的类PLD基因 ,它编码磷脂酶D。在植物体中 ,这个酶是负责干旱诱导降解细胞膜磷脂的主要酶。克隆的PLD1片段有 1 0 69个核甘酸对长。推导的氨基酸序列与已知的PLD基因有很高的同一性 ,包括相似的保守序列特征 ,比如两个HXKXXXXD基元。对花生PLD基因特性需要从遗传和生理上作进一步研究 。

Preharvest aflatoxin contamination has been identified by the peanut industry as the most serious challenge facing the industry worldwide. Drought stress is the most important environmental factor exacerbating Aspergillus infection and aflatoxin contamination in peanut. Development of resistant peanut cultivars would represent a major advance for the U.S. peanut industry. In this study, we identify a novel PLD-like gene, encoding a putative phospholipase D, a main enzyme responsible for the drought-induced degradation of membrane phospholipids in plants. This cloned PLD1 fragment has 1069 bp nucleic acids and the deduced amino acid sequence shows high identity with known PLD genes, having similar conserved features, such as two HXKXXXXD motifs. Further study is needed to genetically and physiologically characterize the PLD in peanut and to gain a better understanding of its function and relationship with drought-tolerance.