中国大青杨基因资源研究

Study on Gene Resources in Populus ussuriensis

收集我国东北林区特有乡土树种大青杨天然基因资源 4 0 0多份 ,在黑龙江省伊春带岭建立了我国第一个大青杨基因库。利用常规手段对其生长、物候、抗锈病和木材材性等性状进行了遗传分析 ,利用高新技术方法从 DNA分子水平上探测了大青杨天然群体的遗传结构和分化程度。经综合评价选出 5个优良群体 ,4 4个优良单株 ,为该地区营建大青杨人工林奠定了一定基础 ,为杨树抗性育种等提供了育种材料和信息。

More than 400 native gene resources of Populus ussuriensis Kom., the special indigenous tree species of northeast forestry region of China, were collected. In 1994 the first gene pool of P. ussuriensis in China was established by these materials in Dailing, Heilongjiang Province.The conventional methods were used to analyze and measure the characters such as growth, phenology, disease resistance to Melampsora larici populina and wood properties,and RAPD analysis was applied to detect the genetic structure of nature populations and differentiation degree on DNA molecular level. Based on the synthetic evaluation, 5 elite populations and 44 elite individuals, which were selected, will provide a basis of establishing plantation in northeast China and the materials and information to resistant breeding in Populus. The result showed that elite individual selection within the best seed sources is an important way of genetic improvement of P.ussuriensis in the near future.