拮抗细菌B-916对水稻植株的抗性诱导作用

The inducing resistance effect of antagonistic bacterium B-916 on rice plant

选择苯丙氨酸解氨酶 (PAL)、超氧化物歧化酶 (SOD)、过氧化物酶 (PO)和多酚氧化酶 (PPO)作为植物抗病性反应的指标 ,对接种纹枯病菌 (RH 2 )和拮抗细菌 (B 916)后水稻植株体内这些酶的活性水平进行比较 ,结果表明 ,接种拮抗菌B 916、病原菌RH 2及同时接种B 916和RH 2 ,都是在接种 2 4hr后PAL和SOD活性最先达到最大值 ,PO在 4 8hr后活性达到最高峰 ,PPO则在 72hr出现高峰值。这些酶活性高峰出现的早迟可能与它们在植物体内所起作用的时间有关。比较不同接种处理诱导产生各种酶活性的强弱可发现 ,接种拮抗菌B 916后诱导产生的PAL和SOD活性数量与接种RH 2 +B 916和接种RH 2的没有明显的差异 ,而接种B 916诱导生产的PO和PPO活性数量要比接种R +B和接种RH 2的少一些。叶面喷施拮抗菌B 916后可以诱导水稻植株内一些与抗病性有关的酶类活性大幅度提高 ,从而增加了水稻抵御病原菌侵入。

Phenlalanine ammonia lynse (PAL),peroxidase (PO),polyphenoase (PPO) and superoxide dismutase (SOD) of rice plants were analyzed as the indicators of rice resistance to diseases,after inoculated with R solani or/and B-916.The results showed that there were different responses of PAL,PO,PPO,and SOD for the injection and the level.PAL and SOD activity peaks reached at 24hr after inoculation,and PO activity peak at 48hr and PPO activity peaks at 72hr.The time of 4 enzymes activity peak was related to their functioning time in rice.There were no difference at activity levels of PAL and SOD after inoculated with RH-2 or B-916,but the activity peaks of PO and PPO inoculated with RH-2 were higher than that inoculated with B-916,which partly indicated that rice plants did not cause disease lesins after inculated with B-916.Therefore,after spraying B-916 on leaves,rice plants were induced to increase the activity levels of 4 enzymes,and enhanced the ability of resistance to rice pathogen infection.