恶性脑肿瘤中CD44内含子9及CD44v6 mRNA的表达水平及临床意义

Levels and clinical significance of CD44 (intron 9) and CD44v6 mRNA in malignant brain neoplasm

目的 了解CD4 4内含子 9、CD4 4v6mRNA在恶性脑肿瘤中的表达水平及临床意义。方法 应用RT PCR技术检测 2 8例恶性脑膜瘤 ,2 5例脑神经胶质瘤及 2 0例脑瘤旁组织中CD4 4内含子 9、CD4 4v6mRNA的表达并对mRNA含量进行半定量分析。 结果 CD4 4v6mRNA在恶性脑膜瘤和脑神经胶质瘤的阳性表达率分别为 92 9% ( 2 6/ 2 8)和 88 0 % ( 2 2 / 2 5 ) ,明显高于瘤旁组织的 5 %( 1/ 2 0 ) ,差异有非常显著性意义 (P <0 0 1) ,前二者相对含量 (mass)值明显高于瘤旁组织。恶性脑膜瘤和脑神经胶质瘤CD4 4内含子 9mRNA表达水平明显高于瘤旁组织 (P <0 0 1)。 结论 CD4 4内含子 9、CD4 4v6mRNA对辅助诊断恶性脑肿瘤有一定的意义。

Objective To investigate the expression of CD44(intron 9) and CD44v6 in brain neoplasm.Methods CD44 (intron 9) and CD44v6 were detected in malignant meningiana, cerebral glioma, and normal brains tissues around the noeplasm respectively by using RT PCR. Results The positive expression rate of CD44v6 mRNA was 92 9%(26/28) in malignant meningioma and 88 0%(22/25) in cerebral glioma. They were significantly higher than 5 0% (1/20) in normal brain tissues ( P <0 01). Mass values of malignant meningioma and cerebral glioma were higher than that of normal brains tissues ( P <0 01). The expression of CD44(intron 9) mRNA was higher in malignant meningioma and cerebral glioma than in normal brains tissues ( P <0 01). Conclusion Detection of CD44 (intron 9) and CD44v6 mRNA might be helpful to the diagnosis of malignant cerebroma.