抑癌基因ING1在鼻咽癌中的表达及突变分析

Expression and Mutation Analysis of ING1 in Nasopharyngeal Carcinoma

本研究运用免疫组化技术、逆转录 - PCR( RT- PCR) ,PCR- SS-CP技术对鼻咽癌组织及鼻咽癌细胞系中 ING1的表达和突变进行检测 .结果发现 3 0例鼻咽癌标本中 ING1蛋白表达下调率为 70 % ( 2 1/ 3 0 ) ;ING1m RNA表达明显下调率为 4 6% ( 12 / 2 6) ,6例鼻咽癌细胞系中有 2例鼻咽癌细胞系表达明显下调 ,而 11例 ( 4 2 .5 % ) NPC组织及其它 4例细胞系表达与正常水平相当 ,另有 3例 ( 11.5 % )鼻咽癌组织过表达 ;单链构像多态性分析 ( SSCP)在鼻咽癌及鼻咽癌细胞系中均未发现突变 .结果表明ING1基因的转录及翻译表达水平与鼻咽癌的发生 /发展呈负相关 。

In order to investigate protein, mRNA expression of ING1 gene in nasopharyngeal carcinoma(NPC),a series of study was carried out by immuno histochemistry, RT PCR and PCR SSCP. The frequency of ING1 protein low expression is 70%(21/30) in NPC; the frequency of ING1 mRNA low expression is 46%; No mutation is found by SSCP analysis in NPC and NPC cell lines. The mRNA and protein expression levels of ING1 gene are negatively correlated with NPC. The expression abnormalities but not mutation of the ING1 are frequent events related to NPC.