壳聚糖对氨基酸-茚三酮体系的荧光增强作用

Fluorescence enhancement of ninhydrin-amino acid in the presence of Zn^(2+) and chitosan

为提高氨基酸分析的灵敏度 ,应用荧光光度法研究了在锌离子的存在下壳聚糖对氨基酸 -茚三酮体系的荧光增强作用 ,探讨了荧光增强作用中金属离子和壳聚糖浓度的影响及其生成配合物的荧光增强作用的机理及条件 ,获得了满意的结果。

Studies have been carried out on the fluorescence enhancement of ninhydrin-amino acid in the presence of Zn 2+ and chitosan. Experimental results show that in the presence of Zn 2+ , chitosan can react with the Zn 2+ -ruhemann′s purple and form a complex, which can enhance the fluorescence of ninhydrin-amino acid system. The machanism of enhancemant and the experimental condition are dicussed also.