卵巢肿瘤端粒酶活性及其催化亚基hTERT mRNA表达的相关性研究

Correlation Study of Telomerase Activity and Expression of hTERT mRNA in Patients with Ovarian Tumor

目的:研究端粒酶激活及其催化亚基的表达在卵巢肿瘤的发生、发展中的意义。方法:采用端粒重复序列扩增-酶联免疫吸附法(Trap-ELISA)及逆转录-聚合酶链反应(RT-PCR)测定端粒酶活性并观察hTERT mRNA表达。结果:3株卵巢癌细胞系端粒酶活性均呈高水平表达,同时hTERT mRNA亦呈高表达;端粒酶阳性率在癌、交界性卵巢肿瘤中分别为74.19％、2/4,在良性及正常卵巢组织中未检测出活性。各组间有显著性差异(P<0.01)。hTERT mRNA仅卵巢癌中表达,阳性率为61.79％;端粒酶阳性率及hTERTmRNA表达率均随手术分期的增高而增高(P<0.01)、卵巢肿瘤端粒酶阳性率及hTERT mRNA表达率呈正相关。结论:端粒酶的激活及其催化亚基的表达与卵巢癌的发生和进展密切相关,二者有望成为卵巢癌预后判断的新指标及基因治疗的新靶点。

Objective: To evaluate the significance of telonierase activity and expression of hTERT mRNA in occurrence and progression of ovarian minor. Methods: The telonierase activity was measured semi-quantitatively by telomeric repeat amplifi-cation protocol (TRAP), and the hTERT mRNA was detected by RT-PCR. Results: The high levels of telomerase activity and hTERT mRNA expression were detected in SKOV3, COC1 and COC2. The positive rates of telomerase activity in ovarian carcinoma, borderline ovarian tumor and benign tumor or normal ovary tissue were 74.91 % , 50 % and 0 respectively (P< 0.01). The hTERTmRNA expressed only in ovarian carcinoma, and the positive rate of its expression was 61.79% . The positive rates of telomerase activity and hTERT mRNA expression were associated with FIGO stage, and they were positively correlated each other. Conclusion: The telomerase activity and hTERT mRNA expression might play an important role in oncogenesis of ovarian tumor and will be a new marker to evaluate prognosis and a new target of gene therapy for patients with ovarian cancinoma.