缺血性脑卒中患者血浆溶血磷脂酸水平的初步研究

Level of plasma lysophosphatidic acid in patients with cerebral ischemic attack, a preliminary study

目的 探讨短暂性脑供血不全发作 (TIA)和脑血栓形成以及疑似脑供血不全发作患者血浆溶血磷脂酸水平的变化。方法 采用有机溶剂抽提 ,并进一步分离出溶血磷脂酸 ,以定磷方法测定。结果 TIA和脑血栓形成以及疑似脑供血不全发作患者血浆溶血磷脂酸水平均显著高于对照组 (1 0 0 μmol/L± 0 70 μmol/L) ,分别为 2 14 μmol/L± 1 0 2 μmol/L、1 5 0 μmol/L± 0 85 μmol/L、1 73μmol/L± 1 14 μmol/L血浆 (P <0 0 5 )。经抗血小板聚集药物或抗凝血药物治疗患者血浆溶血磷脂酸水平下降。结论 作为体内凝血和血栓形成过程早期释放的分子标记物 。

Objective To study the level of plasma lysophosphatidic acid (LPA) in patients with TIA, cerebral thrombosis or possible ischemic attack. Methods Phospholipid was extracted from plasma of patients with TIA, cerebral thrombosis or possible ischemic attack by organic solvent. Lysophosphatidic acid was isolated further. The quantity of LPA was determined by assaying its phosphorous component. Results LPA level of patients with TIA, cerebral thrombosis or possible ischemic attack were 2 14±1 02, 1 50±0 85, and 1 73±1 14 μmol/L blood plasma respectively, all significantly higher than that in control group (1 00±0 70 μmol/L, P <0 05). The plasma LPA level decreased after anticoagulant treatment. Conclusion As a molecular marker released by activated platelets in vivo, plasma PLA may be valuable in diagnosis of cerebral ischemic attack.