原位双杂交法研究Rb、p16^(ink4)基因在非小细胞肺癌中的转录

Study on the transcription of retinoblastoma and p16^(INK4) gene in non -small cell lung cancer by in situ hybridization of double-target mRNAs

目的 :探讨非小细胞肺癌组织中Rb、p1 6INK4 基因的转录。方法 :分别以Bio标记RbcDNA探针、Dig标记p1 6INK4 cDNA探针 ,进行mRNA原位双杂交。结果 :以Bio 辣根过氧化物酶 AEC系统检测Rb基因转录 ,阳性结果为红色 ,阳性率为 83 9% (2 6/3 1 ) ;以Dig 碱性磷酸酸酶 NBT/BCIP系统检测 p1 6INK4 基因转录 ,阳性结果呈蓝色 ,阳性杂交率为 77 4% (2 4/3 1 )。结论 :mRNA原位双杂交法具直观、适合少量细胞、能排除癌组织中混有间质细胞的影响、过程简便等优点 ,Rb、p1 6INK4

Objective:To study the transcription of Rb and p16 INK4 gene in non small cell lung cancer.Methods: In situ hybridization of double targets mRNAs was established with bio labeled Rb cDNA probe and Dig labeled p16 INK4 cDNA probe.Results:Positive in situ hybridization of Rb mRNA showed red,positive in situ hybridization of p16 INK4 mRNA showed blue.The positive ratio of Rb and p16 INK4 gene transcription were 83.9(26/31) and 77.4%(24/31) respectively.Conclusion:The data indicates that in situ double hybridization of double targets mRNAs has the advantage of direct observation,simple approach,exclusion of contamination by interstitial cells.Retinoblastoma and p16 INK4 gene are synergistic and co regulative in the progression of lung cancer