利用羽髓组织代替血清对朗德鹅前白蛋白多态性的研究

Studies on Prealbumin Polymorphisms of Landaise Geese Using Feather Pulp Instead of Serum Sample

以生长羽毛的羽髓组织为试材 ,用聚丙烯酰胺凝胶电泳法研究了朗德鹅的前白蛋白多态性。结果表明 ,用羽髓组织代替血清分析蛋白质多态性是一种较理想的方法 ,且对家禽无伤害。朗德鹅的前白蛋白区电泳图谱存在4～5条谱带 ,分为3个亚区。Paf和Pam区均为一条谱带 ,Pas区为多态 ,发现有Pas1-2和Pas1-1两种表现型 ,其中Pas1 -2占83.33% ,Pas1 -1占16.67%。

Feather pulp was analyzed by means of polyacrglamide gel electrophoresis for Landaise geese's prealbumin polymorphisms. The result showes that the method is recommendable for the study on protein polymorphisms of poultry, and harmless to bird. The electrophoretogram on prealbumin region exists 4～5 prealbumin bands, which is divided by 3 subregions. Paf and Pam are all exist 1 band. 2 phenotypes are found in Pas region. The frequency of type Pas1-2 is at most, reached 83.33%, and 16.67% for that of type pas1-1.