血红蛋白在双十二烷基二甲基铵-聚乙烯硫酸盐多双层复合薄膜电极上的电化学与电催化

Electrochemistry and Electrocatalysis with Hemoglobin at Didodecyldimethylammonium Polyvinyl Sulfate (2C_(12) N^+PVS^-) Multibilayer Composite Film Electrodes

将Hb涂布到由双十二烷基二甲基铵 聚乙烯硫酸盐 (2C12 N+ PVS-)在热解石墨 (PG)电极表面形成的薄膜上 ,即可形成稳定的Hb 2C12 N+ PVS-薄膜。在pH 5 .5的缓冲溶液中 ,该薄膜电极在 - 0 .18V(vs.SCE)左右有一对相当可逆的循环伏安还原氧化峰 ,为Hb血红素辅基Fe /Fe 电对的特征峰 ,在 - 1.0 8V左右HbFe 被进一步还原成HbFe 。在 2C12 N+ PVS-薄膜的微环境中 ,Hb与PG电极之间的电子传递得到极大促进 ,并显示了较好的稳定性。应用所建立的薄层电化学模型和E0′分散模型 ,对pH 7.0缓冲溶液中Hb 2C12 N+PVS-薄膜电极的方波伏安图进行非线性回归拟合处理 ,获得该体系平均式量电位E0′和表观异相电子传递速率常数ks。Hb 2C12 N+ PVS-薄膜可以催化还原氧和某些有机卤化污染物 ,如 :三氯乙酸 (TCA)

Stable thin films were made from didodecyldimethylammonium polyvinyl sulfate (2C(12)N(+) PVS-) with incorporated hemoglobin (Hb) on pyrolytic graphite (PG) electrodes. Cyclic voltammogram of Hb-2C(12)N(+) PVS- films showed a pair of well-defined and nearly reversible peaks for HbFe(III/Fe(II) couple at about - 0.18 V vs. SCE in pH 5.5 buffers, and at about - 1.08 V HbFe(II) was further reduced into HbFe(I). The electron transfer rate between Hb and PG electrodes was greatly facilitated in microenvironment of 2C(12)N(+) PVS- films, showing better stability. The average formal potential (E-o') and the apparent heterogeneous electron transfer rate constants (k(s)) was obtained at pH 7.0 buffer by nonlinear regression analysis using a model featuring dispersion of formal potentials. Oxygen and organolialide pollutants such as trichloroacetic acid (TCA) were catalytically reduced by Hb-2C(12)N(+) PVS- films.