大豆耐盐性种质的分子标记辅助鉴定及其利用研究

MARKERS-ASSISTED IDENTIFICATION OF THE SALT TOLERANT ACCESSIONS IN SOYBEAN

大豆耐盐种质的鉴定对于促进大豆耐盐育种具有重要作用。本文利用已获得的大豆耐盐性基因的共显性PCR标记 ,对选自于国家作物种质库的 5 9份耐盐和盐敏感种质加以鉴定 ,同时进行了田间耐盐性重复鉴定 ,并对种质库耐盐性记载结果、田间耐盐性重复鉴定结果与分子标记鉴定结果进行比较 ,从中选出田间耐盐性重复鉴定结果与分子标记鉴定结果一致的耐盐种质 4 2份 ,分析了这些种质间的遗传多样性 ,为大豆种质资源的改良及耐盐遗传育种中的亲本选配提供了理论依据 ,同时对分子标记应用于种质鉴定和育种实践进行了有益的尝试。

The identification of soybean tolerant accessions play a important role in soybean breeding. The molecular markers of salt tolerant gene loci were used to identify 59 salt tolerant and susceptible accessions of soybean selected from the National Gene Bank. The 42 tolerant accessions with the same result between the re-identification in the field and the identification with marker were selected out through comparing among the records for salt tolerance in Gene Bank, the re-identification in the field and the identification with marker, and the genetic diversity of these accessions were analyzed. It has provided the valuable information for selecting salt tolerant parent in improving cultivars and generating novel lines in soybean breeding. Meanwhile, it made useful exploration on the application of molecular markers to accessions identification and breeding.