参附汤对失血性休克大鼠肝脏细胞液糖皮质激素受体的影响

Effects of the Shen Fu Tang(SFT) on glucocorticoid receptor(GCR) in the hepatic cytosol in hemorrhagic shock rats

目的和方法：使用放射配体结合法检测正常对照组、失血性休克组、参附汤加失血性休克大鼠肝细胞液糖皮质激素受体（ＧＣＲ）数目。同时检测血浆皮质酮含量（ＣＣ）。结果：参附汤加失血性休克组失血１２ｈ后，肝细胞ＧＣＲ数目明显高于失血性休克组（Ｐ＜０．０１），与正常对照组比较无显著性差异（Ｐ＞０．０５）。失血性休克组及参附汤加失血性休克组ＣＣ无显著性差异（Ｐ＞０．０５），但均高于正常对照组（Ｐ＜０．０１）。结论：参附汤的抗休克作用与其减轻休克时ＧＣＲ的减少且不影响血浆ＧＣ水平，增强ＧＣＲ功能有关。

Aim and Methods: Wistar rats were divided into three group: control, hemorrhagic shock(shock), administration of SFT before and after hemorrhagic shock (SFT+shock). The glucocorticoid receptors(GCR) in the hepatic cytosols of rats in the three groups were measured by radioligand binding assay using [3H] dexamethssone as the ligand. Plasma corticosterone(CC) concentration was determined by competitive protein-binding assay. Results: The specific binding of GCR in the hepatic cytosols in rats 12h after hemorrhage in SFT+shock was higher more than that of rats in shock(P<0.01), and the difference was not significant as compared with the control. The concentration of plasma corticosterone was not altered by SFT. Conclusion: SFT may increase the level of GCR in the hepatic cytosols ,and maintain the plasma corticosterone(CC) concentration at a high level of hemorrhagic shock rats. SFT may be of benefit to the treatment of shock.