NPCLC中P_(16)和Rb基因产物的表达情况及与细胞增殖的关系

Relationship between expression of gene products P_(16) and Rb in non parvicellular lung carcinoma

目的：探讨抑癌基因的Ｐ１６、Ｒｂ基因产物在非小细胞肺癌化Ｓ－Ｐ法，检测６８例ＮＰＣＬＣ 患者的手术切除标本的 Ｐ１６、Ｒｂ的表达情况，并进行ＰＣＮＡ检测，计算细胞增殖指数（ＰＩ， ｐｒｏｌｉｆｅｒａｔｉｏｎ ｉｎｄｅｘ）。结果：在６８例ＮＰＣＬＣ 患者标本中，Ｐ１６、Ｒｂ的阳性率分别为６４．７％、８８．５％，二者在不同组织类型的ＮＰＣＬＣ中的表达无显著性差异（Ｐ＞０．０５）；二者的表达均随ＰＩ分级的升高有不同程度的下降。３１例低度恶性ＮＰＣＬＣ肿瘤中，Ｒｂ均为阳性表达（＋、＋＋），２９例Ｐ１６阳性或强阳性（＋、＋＋）表达；在３７例高度恶性ＮＰＣＬＣ中２９例Ｒｂ阳性或强阳表达，其中Ｐ１６、表达缺失２２例（７５．９％）；而在８例Ｒｂ阴性的ＮＰＣＬＣ中有６例（７５％）为Ｐ１６阳性或强阳性表达。结论：１、Ｐ１６及Ｒｂ参与ＮＰＣＬＣ的细胞增殖过程，并且Ｐ１６蛋白的缺失可能是ＮＰＣＬＣ由低度恶性向高度恶性过渡重要事件；２、Ｐ１６与Ｒｂ在高恶性ＮＰＣＬＣ肿瘤中呈负相关性表达，这种负相关在ＮＰＣＬＣ中是一常见事，是ＮＰＣＬＣ高度恶性的标志之一。

Objective: To study expression of product of genes p16 and Rb in non parvicellular lung carcinoma (NPCLC), and relation ship between genes P16 and Rb and cell proliferation. Methods: Using S-P immunohistochemical methods, the expressions of P16\ Rb and PCNA protein in 68 cases of NPCLC were studied ,and the proliferation index(PI) was calculated. Results: The positive rates of P16 and Rb were 64.7%and 88.5% in 68 cases of NPCLC, with no significant difference in different histological types of NPCLC (P>0.05). The P16 protein expression was different in different PI grading, with the P16 protein expression lower and the PI grading higher, and the Rb expression was lower with the rising PI grading. The Rb expression was all positive ,and P16 showed positive expression in 29 of 31 low magilant NPCLC. The positive expressions of Rb and P16 were showed in 29 and 15 cases respectively in 37 high magilant NPCLC; Six showed positive or strong positive or strong positive expression in 8 cases of Rb negative expression. Conclusion: 1.P16 and Rb take part in process of cell proliferation of NPCLC, and the loss of P16 protein is important from low to high magilant changes in NPCLC . 2. P16 and Rb are negatively correlative expression in high magilant NPCLC, and this correlation is common and may be one magilant sign in NPCLC.