全反式维甲酸预防后囊膜混浊的研究

Study on Prevention of Posterior Capsule Opacification by Retinoic Acid in Vitro

目的 研究在体外细胞培养条件下 ,全反式维甲酸对人类晶体上皮细胞增殖的抑制作用及有效抑制浓度 ,为预防后囊膜混浊发生的研究提供线索。方法 传代培养的人类晶体上皮细胞经计数后在 37℃ ,5 %CO2 的条件下培养 4 8小时 ,再加入浓度分别为 2 ,5 ,10 ,15 ,2 0 μg/ml全反式维甲酸完全培养基作为实验组 ,继续培养 2 4及 72小时后进行活细胞计数并与所设空白对照组对比 ,观察全反式维甲酸对传代细胞的抑制作用并找出有效作用浓度及半效抑制量 (ID50 )。同时观察培养 2 4小时后全反式维甲酸对传代细胞贴壁的抑制作用。结果 全反式维甲酸对体外培养的人类晶体上皮细胞的增殖有明显的抑制作用。从 2 μg/ml浓度即有显著的抑制效应 ,到 10 μg/ml浓度时达到最大作用。全反式维甲酸作用 2 4小时的ID50 是 9 2 2 μg/ml;作用 72小时的ID50 为 4 75 μg/ml。 结论 全反式维甲酸具有有效地抑制体外培养的人类晶体上皮细胞的增殖和贴壁作用 ,有可能成为预防人类晶体后囊膜混浊的理想药物。

Objective To investigate the inhibitory activity and the effective concentration of all-trans retinoic acid against the proliferation of human lens epithelial cells(HLEC)in vitro,and give a clue to the study on the prevention of posterior capsule opacification.Methods The passage cultured human lens epithelial cells were incubated at the condition of 37℃,5%CO 2 for 48 hours after counted and then exchanged with the medium contained 2,5,10,15,20μg/ml of all trans retinoic acid respectively as the tested groups,which were compared with the blank control group with normal medium.Then these passage cells were counted again after another 24 and 72 hours to analyze the inhibitory function on human lens epithelial cells by all-trans retinoic acid and found out its effective concentration as well as half inhibitory dose(ID 50 ).The inhibition of 24 hours cultured passive HLEC attachment by all-trans retinoic acid were also studied.Results There was a prominent inhibition on the proliferation of HLECs by all-trans retinoic acid:from 2μg/ml until 10μg/ml to reach its peak,the 24 hours ID 50 was 9 22μg/ml and the 72 hours one was 4.75μg/ml.Conclusion All-trans retinoic acid can inhibit the cultured HLEC proliferation and attachment in vitro and may become an ideal agent for the prevention of posterior capsule opacification in human.