空斑减少中和试验测定血清抗乙脑病毒抗体

Plaque Reduction Neutralization Test in Determination of Human Anti-Japanese Encephilitis Antibodies

目的 对比空斑减少中和试验与反向被动血凝抑制试验在评价人血清抗乙脑病毒抗体时测定结果的异同。方法 对 4 5 0名 6～ 10岁儿童接种灭活乙脑疫苗前后的血清样本用两种方法进行了测定。结果 两种方法在测定免前人血清抗乙脑病毒抗体时存在显著性差异 ,在测定免后人血清抗乙脑病毒抗体时两者没有差异。结论 空斑减少中和试验比反向被动血凝抑制试验在测定人血清抗乙脑病毒抗体时灵敏。

Objective In order to evaluate the difference between plaque reduction neutralization test(PRNT) and reverse passive hemagglutination inhibition(RPHI) test on human anti Japanese encephilitis antibodies.Methods Pre-and postimmunization sera collected from 450 children aged 6-10 years old after vaccination with inactivated JE were detected by both methods.Results No significant difference was found between these two methods when they were used to measure the post immunization sera,but significant differnce was found between these two methods when they were used to measure the preimmunization sera.Conclusion PRNT was more sensitive than RPHI when they were used to measure the anti Japanese Encephilitis antibodies.