光质和NAA组合对怀山药叶片脱分化的效应

The Effect of Combination of Lignt Quality and NAA on Dedifferentiation in Leaves of Dioscorea Opposita

不同光质和NAA组合对怀山药叶片脱分化的效应不同 ,结果表明 :各种光质下 ,(1)NAA浓度为2mg·L-1时 ,怀山药叶片愈伤组织形成的时间最早 ;NAA浓度为 2mg·L-1或 4mg·L-1时 ,出愈率最高 .(2 )愈伤组织中的可溶性蛋白含量和过氧化物酶 (POD)活性不同 :蓝光有利于愈伤组织中可溶性蛋白的形成 。

This paper deals with the effect of combination of light quality and NAA on the dedifferentiation of the bulbil of Dioscorea opposita.Results showed different effect under different light quality as follovos:(1)when the consistency of NAA was 2mg/L,the time of callus formation was earliest;when the consistency of NAA was 2mg/L or 4mg/L,the rate of callus production was the highest.(2)soluble protein of the callus was favourably increased by blue light,but peroxidase(POD)activity of the callus was advanced by yelow light.