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哺乳动物显微注精的研究

Progress of mammalian sperm microinjection
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摘要 显微注精过程主要包括固定针和注射针的制备 ,精子的分离 ,卵母细胞的采集 ,卵母细胞的激活和培养 ,胚胎移植。显微注精不需要精子结构上的完整性 ,精子核的去凝聚与二硫键有关 。 The main technological procedure of sperm microinjection includes preparation of oocyte-holding and injection pipette,isolation of sperms,collection of oocytes,sperm microinjection,activation and culture of oocytes,embryo transfer.Sperm do not need to be structurally intact for injection.The decondensation of sperm nuclei is related to disulfide bonding and the successful fertilization is dependent on oocyte activation.
作者 征曰良 仲跻锋 宋杰
机构地区 山东师范大学生命科学学院 山东省农科院生物技术研究中心
出处 《生物学杂志》 CAS CSCD 2002年第1期24-26,共3页 Journal of Biology
关键词 显微注精 卵母细胞精子 去凝聚 激活 哺乳动物 sperm microinjection oocyte sperm decondensation activation.
分类号 S814.3 [农业科学—畜牧学] R321－33 [医药卫生—人体解剖和组织胚胎学]
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参考文献12

  • 1[1]Kimura Y, Yanagimachi R. Intracytoplasmic sperm injection in the mouse. Biol Reprod. 1995, 52: 709 - 720.
  • 2[2]Kuretake S, Kimura Y, Hoshi K et al. Fertilization and development of mouse oocytes injected with isolated sperm heads. Biol Reprod. 1996, 55:789 - 795.
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  • 8[8]Kimura Y, Yanagimachi R. Mouse oocytes injected with testicular spermatozoa or round spermatids can develope into normal offspring. Development. 1995,121:2397 - 2405.
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生物学杂志
2002年 第1期

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