脱氧核酶研究进展

Progress on Deoxyribozymes

使用体外分子进化技术 ,从一个人工合成的随机多核苷酸单链DNA库中筛选出具有酶活性的DNA分子 ,称为脱氧核酶 .目前已经筛选出具有RNA切割作用、DNA切割作用、金属螯合作用和过氧化物酶活性、DNA激酶活性以及DNA连接酶活性等多种催化功能的脱氧核酶 .特别是脱氧核酶 10～ 2 3,无论在体外应用于RNA限制性内切酶 ,还是在生物系统内作为RNA水平上的基因失活剂 ,都具有很好的应用前景 .

DNAs with catalytic potential isolated from a ssDNA pool with random-sequence in vitro selection, were called deoxyribozymes or DNAzyme. Deoxyribozymes that exhibit RNA transesterification, DNA transesterification, porphyrin metalation and peroxidase activities, DNA kinase activity and DNA ligase activity have recently been reported. Deoxyribozyme 10similar to23 might act as a sequence-specific RNA endoribonuclease in vitro, and could be used to inactivate target cellular RNA in vivo.