胃肠富集Kruppel样因子在食管癌的表达

Expression of Gut-enriched Kruppel-like Factor in Esophageal Squamous Cancer

胃肠富集Kruppel样因子 (GKLF)是一个新近发现的真核锌指蛋白 ,它在胃肠道表达丰富 ,其表达与细胞生长停滞有关联 .用半定量的RT PCR的方法 ,比较了食管鳞癌病人癌组织和正常粘膜的GKLF表达 .17例食管鳞癌病人均检测到GKLFmRNA的表达 ,其中 14例癌组织中GKLF表达比临近正常组织减少 .人原代培养成纤维细胞中GKLF的去血清诱导作用明显 ,而在一食管鳞癌细胞系EC970 6中该诱导作用减弱 .对EC970 6细胞GKLFcDNA的序列分析表明 ,该基因的cDNA编码区未发生突变 .结果证实 ,食管鳞癌中GKLF表达下调 .

Gut-enriched Kruppel-like factor ( GKLF) is a newly identified eukaryotic zinc finger protein expressed extensively in (tie gastrointestinal tract, the expression of which is associated with growth arrest. GKLF expression in the tumor was compared to normal mucosa in esophageal squamous cancer patients, using semi-quantitative RT-PCR. Expression of GKLF mRNA was detected all of 17 cases of esophageal squamous cancer. Decreased expression of GKLF mRNA was observed in 14 of esophageal squamous cancer patients compared to normal esophageal tissues adjacent to tumor from the same patient. Serum deprivation induced overexpression of GKLF was observed in the primary culture of human fibroblast and this induction was decreased in an esophageal squamous cancer cell line EC9706. Sequencing of GKLF cDNA from EC9706 showed no mutations in the coding region. The results demonstrate that GKLF expression in esophageal squamous cancer is down-regulated.