小麦硫代硫酸硫转移酶类似基因的克隆与定位(英文)

Molecular Cloning, Characterization and Mapping of a Rhodanese Like Gene in Wheat

小麦 簇毛麦 6VS/6AL易位系 92R137含有抗白粉病基因Pm2 1。为了研究该易位系的抗病机理 ,应用mRNA差异显示和快速扩增cDNA未端 (RapidAmplificationofcDNAEnd ,RACE)技术对在白粉菌诱导后表达增强的基因进行了克隆。分离到 1个命名为TaTST的全长cDNA序列。Northern杂交分析表明 ,TaTST基因在白粉菌诱导后表达明显增强 ,2 4h达到峰值。氨基酸序列同源性分析表明 ,TaTST与Datiscaglomerata的硫代硫酸硫转移酶基因(rhodanese,EC ,2 .8.1.1)序列有 6 4 %相同 ,80 %相似。用中国春缺体 /四体系和端体系Southern杂交和基因特异性引物扩增 (genespecificprimer PCR)将TaTST基因定位在小麦 6B染色体短臂上。Southern杂交表明 ,该基因为单拷贝基因。由于在杨麦 5号和 6VS/6AL易位系间存在明显多态 ,可以推测在 6VS上有TaTST的同源基因。TaTST是从小麦中分离的新基因。白粉菌诱导后的表达变化提示 :TaTST与小麦抗白粉病反应有关。

WT10.5,10.25BZ]To isolate genes related to resistance to Erysiphe graminis (Blumeria graminis ) DC. f. sp. tritici in wheat ( Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from seedlings of a wheat Haynaldia villosa 6VS/6AL translocation line 92R137 that contains a powdery mildew resistance gene Pm21 . A full length cDNA sequence named TaTST (Triticum aestivum thiosulfate sulfurtransferase) homologous to the thiosulfate sulfurtransferase (rhodanese) in Datisca glomerata was isolated. Northern blot showed that the expression of TaTST was enhanced after infection with Erysiphe graminis. TaTST was mapped on the short arm of 6B chromosomes of wheat through Southern blot and GSP PCR using Chinese Spring nullisomic/tetrasomic lines and ditelosomic lines. There is a homologue of TaTST on 6VS too.