黄芪对豚鼠Ⅰ型超敏反应的影响

INFLUENCE OF MEMBRANOUS MILKVETCH ROOT ON GUINEA PIG OF TYPE I HYPERSENSITIVITY

目的 :观察黄芪对豚鼠 型超敏反应早、晚期及细胞因子的影响。方法 :以马血清致敏豚鼠作为 型超敏反应的动物模型。结果 :通过病理观察发现 ,中药黄芪既能抑制毛细血管扩张、通透性增强、腺体分泌增加、平滑肌收缩等超敏反应的早期病理改变 ,也能抑制以嗜酸性粒细胞浸润为主的晚期病理改变 ,效果显著。应用 EL ISA检测 IL- 4、IFN- 在豚鼠血清中的含量。致敏组和实验用药组 IL- 4浓度均值为31.2 2 pg/ m l和 14.40 pg/ m l(P<0 .0 5 ) ,而 IFN- 的均值分别为 10 .32 pg/ ml和 2 3.14pg/ m l(P<0 .0 5 )两者差异均有显著性。结论 :黄芪可能是通过调整了 Th1和 Th2的偏移 ,使 IL - 4分泌减少 ,B细胞合成的Ig E也随之明显降低 ,从而阻止了 型超敏反应的发生。

Guinea pig sensitized by horse serum was used as animal model of type I hypersensitivity.Through pathological observation,we found that traditional Chinese medicime membranous milkvetch root could inhibit early-phase reaction,including capillary dilatation,vascular permeability and gland secretion increase,smooth mucle constriction ,and so on.Meanwhile it conld inhibit late-plasereaction which was characterized with ifiltrationof eosinophil.The effect was dramatic.We detected the concentration of IL-4 and IFN- in serum of guinea pig by ELISA.The average of IL-4 in sensitized group and medicine group were 31 22 pg/ml and 14 40 pg/ml (P<0 05) respectively,while the average of IFN-r were 10 32 pg/ml and 23 14 pg/ml(P<0 05).The difference between the two groups was obvious.This suggested that membranous milkvetch root probablyreduced the secretion of IL-4 by regulating the shift of Th1 and Th2,acordingly IgE synthesized by B cells decreased.So membranous milkvetgh root could prevent from type I hypersensitivity.