摘要
ToRCH系列病原微生物(包括弓形虫Tox、风疹病毒RuV、巨细胞病毒CMV、单纯疱疹病毒HSV Ⅰ/Ⅱ等)是一组具有致畸作用的病原体,孕妇感染可导致流产 、早产、畸形甚至死胎。目前ToRCH IgM型抗体的检测都在不同程度上受到非特异性的干扰,采用有效方法消除干扰对ToRCH IgM型抗体检测的准确性至关重要。为此我们对比了多种消除IgM检测中非特异性干扰 的方法,并以最佳方法对256例女性献血者、143例正常妊娠和61例异常妊娠标本进行ToRCH IgM抗体的对比检测。结果显示健康人群中ToRCH活动性感染率较高,健康孕妇ToRCH IgM的 总阳性率高达23.1%(其中Tox5.6%、CMV9.8%、RuV8.4%、HSV4.9%);而异常妊娠则显著上升 ( p<0.01),其中Tox、CMV、RuVIgM阳性率上升明显(p<0.05),分别为19.7%、26.2%、24.6% 。 由此可见孕期ToRCH病原微生物活动感染是致异常妊娠的主要因素之一,应严密监视孕期ToR CH病原微生物活动性感染,特别是弓形虫、风疹病毒和巨细胞病毒的感染。
To research ToRCH IgM clinic diagnosis,four kinds for met h ods for non-specific interference removed have been compared in IgM detection;t h en ToRCH IgM in sera of 256 female donors,143 normal pregnant women and 61 abnor mal pregnant women were detected with the best method,anti-human IgG(γ chain s pecific),resolving non-specific interference.The results show that the positive rate of ToRCH IgM in normal population was very high,it was up to 23.1% in hea l th pregnant women (Tox 5.6%,CMV9.8%,RuV8.4%,HSV4.9% respectively),while it incre ased dramatically in abnormal pregnant women (p<0.01),so did Tox,CMV,RuV IgM(9.7 %,26.2%,24.6% respectively).Then ToRCH pathogen active infection during pregnanc y can cause abnormal pregnancy and should be inspected carefully,esp.Tox,CMV,RuV .
出处
《中国病毒学》
CSCD
2002年第1期56-59,共4页
Virologica Sinica