抗ToRCH-IgM型抗体临床对比检测研究

Experimental Studies and Comparison on Clinic Diagnosis of ToRCH IgM Antibody

ＴｏＲＣＨ系列病原微生物（包括弓形虫Ｔｏｘ、风疹病毒ＲｕＶ、巨细胞病毒ＣＭＶ、单纯疱疹病毒ＨＳＶ Ⅰ／Ⅱ等）是一组具有致畸作用的病原体，孕妇感染可导致流产 、早产、畸形甚至死胎。目前ＴｏＲＣＨ ＩｇＭ型抗体的检测都在不同程度上受到非特异性的干扰，采用有效方法消除干扰对ＴｏＲＣＨ ＩｇＭ型抗体检测的准确性至关重要。为此我们对比了多种消除ＩｇＭ检测中非特异性干扰 的方法，并以最佳方法对２５６例女性献血者、１４３例正常妊娠和６１例异常妊娠标本进行ＴｏＲＣＨ ＩｇＭ抗体的对比检测。结果显示健康人群中ＴｏＲＣＨ活动性感染率较高，健康孕妇ＴｏＲＣＨ ＩｇＭ的 总阳性率高达２３．１％（其中Ｔｏｘ５．６％、ＣＭＶ９．８％、ＲｕＶ８．４％、ＨＳＶ４．９％）；而异常妊娠则显著上升 （ ｐ＜０．０１），其中Ｔｏｘ、ＣＭＶ、ＲｕＶＩｇＭ阳性率上升明显（ｐ＜０．０５），分别为１９．７％、２６．２％、２４．６％ 。 由此可见孕期ＴｏＲＣＨ病原微生物活动感染是致异常妊娠的主要因素之一，应严密监视孕期ＴｏＲ ＣＨ病原微生物活动性感染，特别是弓形虫、风疹病毒和巨细胞病毒的感染。

To research ToRCH IgM clinic diagnosis,four kinds for met h ods for non-specific interference removed have been compared in IgM detection;t h en ToRCH IgM in sera of 256 female donors,143 normal pregnant women and 61 abnor mal pregnant women were detected with the best method,anti-human IgG(γ chain s pecific),resolving non-specific interference.The results show that the positive rate of ToRCH IgM in normal population was very high,it was up to 23.1% in hea l th pregnant women (Tox 5.6%,CMV9.8%,RuV8.4%,HSV4.9% respectively),while it incre ased dramatically in abnormal pregnant women (p<0.01),so did Tox,CMV,RuV IgM(9.7 %,26.2%,24.6% respectively).Then ToRCH pathogen active infection during pregnanc y can cause abnormal pregnancy and should be inspected carefully,esp.Tox,CMV,RuV .