桃蚜体内与病毒结合的共生菌Buchnera　groEL基因的克隆和序列分析

Cloning and Sequence Analysis of Buchnera GroEL Gene from Endosybiotic Bacterium of Myzus persicae

以桃蚜（Ｍｙｚｕｓ ｐｅｒｓｉｃａｅ）杨凌生物型为材料，利用一对特 异性引物 ，用ＰＣＲ的方法从桃蚜体内扩增出了内共生菌的Ｂｕｃｈｎｅｒａ ｇｒｏＥＬ基因，序 列 测定结果表明其长度为１ ６４７ｂｐ，与ＧｅｎＢａｎｋ中的桃蚜荷兰生物型、蜿豆蚜（Ａｃｙｒｔｈｏｓｉｐｈｏｎ ｐｉｓｕｍ）日本生物型Ｂｕｃｈｎｅｒａ ＧｒｏＥＬ基因长度相同，同源性分别为９９％和９１％；Ｂｕｃｈｎｅｒａ ＧｒｏＥＬ－ＹＬ编码５４８个氨基酸，序列比较发现与Ｂｕｃｈｎｅｒａ ＧｒｏＥＬ－ＮＴ仅有３个氨基酸的差异，即ＡＡ１１１Ｍｅｔ→Ｌｙｓ、ＡＡ２２２Ｖａｌ→Ｍｅｔ ａｎｄ ＡＡ３４８Ｇｌｎ→Ｈｉｓ．

Buchnera groEL gene of endosymbiotic bacterium (Buc hnera sp.) from Myzus persicae Yangling boitype (P.R.China) was isloated,a n d the complete nucleotides sequence was determined.It is indicated that Buchne ra groEL gene of M.persicae includes 1647 base pairs,has 99% and 91% sequ ce similarity with Buchnera groEL gene of M.persicae The Nether land bi otype (AF003957) and Acyrthosiphon pisum Japanese biotype (X61150),respectiv ely.Buchnera GroEL-YL contains 548 amino acide,and has 3 different amino ac ids comparing with Buchnera GroEL-NT,that is AA111Met→Lys、AA222Val→Met and AA348Gln→His.