猪瘟病毒野毒株持续感染细胞模型的建立

Model Establishment of the Cell Persistently Infected bya Field-isolated Virulent Strain of CSFV

采用猪瘟病毒野毒珠（ＣＳＦＶ３９）感染猪肾传代细胞系ＰＫ－１５，经连续传７９代，建立了稳定的病毒持续感染细胞模型，获得ＣＳＦＶ３９－ＰＫ１５细胞株。用免疫荧光、ＲＴ－ＰＣＲ检测、透射电镜跟踪观察了ＣＳＦＶ３９－ＰＫ１５细胞株连续传代中病毒在细胞内的存在情况。结果表明第９，２９，７９代细胞仍有猪瘟病毒存在，表现出病毒持续感染的基本特征。这为深入研究猪瘟病毒持续感染机理奠定了基础。

A porcine kidney cell line(PK-15) was infected by a fiel d-isolated virulent strain of classical swine fever virus (CSFV39).After the in f ected cells had subcultured 79 passages for near eight months,a steady m odel of the cell persistently infected by virus has been established,hence the C SFV39-PK15 cell line was procured.In this experiment immunofluorescence,RT-PCR assa y and electron microscopy observation were used to identify the basic viral feat ures in the CSFV39-PK15 cell. The results showed the 9th,29th,79th CSFV39-PK1 5 cell passages still harbored CSFV and they had the radical characteristics of v iral persistent infection.This endeavor should be the foundation for the furthe r research of CSFV persistent infection.