人体正常组织的流式细胞术DNA含量参数检测

The Measurement of DNA Content Paramenters in Different Tissues of Human Organ

[目的]建立人体各种正常组织的流式细胞术细胞动力学参数。[方法]用流式细胞术检测337例恶性肿瘤或良性疾病患者某些正常组织的DNA指数(DI)、DNA倍体类型、细胞凋亡水平和增殖活性。[结果]在337例患者的正常组织中DI值为1.00±0.00,DNA倍体为2倍体 ,其细胞凋亡水平为2.67%±2.50% ,S期细胞比率为5.00%±4.59 %。在不同类组织中 ,某些组织的SPF检出率有显著性差异(P<0.05或P<0.01)。恶性肿瘤患者正常组织的Apo和SPF均显著高于良性疾病患者的正常组织(P<0.01和P<0.05)。[结论]在肿瘤研究中选择正常对照组织时 ,恶性肿瘤患者的“正常”组织未必能真正代表机体的正常组织 ;而选择良性疾病患者的正常组织作为肿瘤研究的正常对照组织 ,这种组织可能更接近无任何疾病的正常机体组织的生物学特性。

To explore the value of DNA content paramenters in different tissues of human organ.We measured DNA index(DI),DNA ploid type,apoptosis(Apo)level and proliferative activity of different normal tissues from 337 patients with benignly disease or malygnant tumor by frow cytometry.The results showed that DI value,DNA ploid type,percentage of Apo and SPF(S phase faction)of normal tissues from those patients were 1.00±0.00,diploid,2.67%±2.50%,5.00%±4.59%respectively.The percentage of SPF in different normal tissue was significant different among different organs (P<0.01 or P<0.05).The percentage of Apo and SPF in normal tissue in patients with malignant tumor were higher than that of patients with benign disease(P<0.01 or P<0.05).[Conclusion]'Normal tissue' of patients with malignant tumor should not be selected as normal control tissue in carcinoma study.The normal tissues in patients with bening disease may have similar biological characteristics in tissues of healthy human being.