绿僵菌不同菌株DNA多态性的RAPD分析

Analysis on isolates of Metarrhizium anisopliae by RAPD

采用RAPD PCR技术分析了 4个绿僵菌菌株的DNA遗传多态性。从 1 60条引物中筛选出 2 7条引物 ,对各菌株进行PCR扩增 ,结果 4个绿僵菌菌株共扩增出 334个位点 ,其中多态性位点 2 99个 ,占 89 5 % ,表明各菌株间具有较丰富的遗传多态性。菌株DNA多态性与原寄主和孢子形态表现出明显的相关性 ,但与对松墨天牛幼虫的毒力间未表现出相关性。图2表 4参

DNA of Metarrhizium anisopliae isolates was analyzed by RAPD.Twenty seven primers screened and optimized out of 160 primers were applied to the proliferation of PCR.Three hundred four loci have been proliferated in 4 strains of M. anisopliae,299 loci out of which are of polymorphism and account for 89 5%.All these suggest that each strain has an abundant genetic polyorphism.There is some correlation between DNA polymorphism of strains and their original hosts,size of conidiospore,distributions,but no obvious correlation between DNA polymorphism and virulence on Monochamus alternatus larvae.