胚龄和2,4-D浓度对玉米自交系幼胚愈伤组织诱导率的影响

Influence of Embryo Age and 2,4-D Concentration on Callus Induction Ratios in Inbred Lines of Maize

玉米幼胚愈伤组织的诱导由于受多种因素的影响使其相对于其它禾本科植物而言较为困难 ,其中胚龄和 2 ,4_D浓度是影响玉米幼胚愈伤组织诱导的两个较为主要的因素。本文分析了胚龄和 2 ,4_D浓度对玉米幼胚愈伤组织诱导率的影响 ,提出了玉米幼胚组织培养的最适的胚龄因自交系而异 ,对于吉 85 3,吉 842两种自交系胚龄以 11～ 13d为宜 ,而 4112自交系则以 12～ 14d为宜 ;3种自交系诱导的适宜 2 ,4_D浓度都为 1mg/L ,自交系间差异不大。为进一步研究能长期继代的玉米幼胚胚性愈伤组织的诱导 。

The initiation of maize immature embryo is more difficult than other crops because it is influenced by more factors.Besides genotype and physical conditions,the concentration of 2,4_D and embryo age are important factors.In this paper,we analized their influence on the process of initiation,and concluded the proper concentration of 2,4_D and the proper embryo ages of inbred lines of Ji853,Ji842,4112.