香蕉束顶病毒NS株系DNA组分6的克隆及序列分析

Cloning and Sequencing of DNA Component 6 of BBTV NS Strain

通过常规的基因克隆技术 ,对香蕉束顶病毒NS株系代表分离物的DNA组分 6进行了克隆和序列分析 ,并将该分离物这个组分的序列与先前报道的广州天河分离物 (属NSP株系 )的该组分序列进行比较 ,结果表明 :该组分全序列、ORF及其编码的氨基酸序列在 2个分离物间的变异率分别为 3 4%、1 7%和 2 6 % .表明该组分在 2个株系代表分离物间存在较显著差异 ,从而进一步支持了广东BBTV存在 2个株系的结论 .此外 ,NS株系代表分离物DNA组分 6的全序列、ORF及其编码的氨基酸序列与澳大利亚分离物的变异率分别为 14.4%、7.5 %和 7.1% .

By regular gene cloning techniques, DNA component 6 of the representative isolate of BBTV (banan bunchy top virus) NS strain was cloned and sequenced. The sequence was compared with the sequence of DNA component 6 of Guangzhou Tianhe isolate (belong to NSP strain) reported. The results showed that the variance ratios of full sequence of the component 6,the ORF nucleotide sequence and its encoding amino acid sequence between the two isolates were 3 4%, 1.7% and 2.6% respectively.Results in the experiment showed that the differences of the component between the two representative isolates of the NSP and NS strains respectively were significant, and that further supported that there were NSP and NS strains of Guangdong BBTV. In addition, the variance ratios of the full sequences of the component, the ORF nucleotide sequences and its encoding CP amino acid sequences were 14.4%, 7 5% and 7 1% respectively between the Gaozhou isolate and the Australia isolate.