胡萝卜抗HgCl_2变异细胞系的筛选

Selection of the Carrot Variant Cell Line Resistant to HgCl_2

利用胡萝卜下胚轴愈伤组织 ,以HgCl2 为选择剂 ,通过多步正筛选法 ,获得了抗性变异细胞系 .抗性鉴定结果表明 ,在不同HgCl2 浓度的培养基上 ,抗性系的愈伤组织存活率和鲜质量增殖率均明显高过亲本系 ,抗性系的细胞膜透性也发生了变化 .对抗性系和亲本系愈伤组织进行再分化的结果表明 ,在连续 3～ 4代的分化培养之后 ,愈伤组织都能再生绿苗 .提出了该项研究在预防和治理重金属污染方面的重要意义 .

The carrot variant cell line resistant to HgCl 2 was selected through multi step selection from even and stably growing calli of carrot hypocotyl. The result of resistance identification showed that when adding different concentration of HgCl 2 to the culture medium, the calli survival ratio and fresh weight increasing percentt of the resistant line were significantly higher than those of the parental line , and the cell membrance permeability of the resistant line changed too. The callus differentiation result showed both the parental line and the resistant line regenerated green shoots from the long term subcultured calli through 3-4 generations differentiation. And the important of this study to prevent and control environmental contaminations was put forward.